Generation 2  hardware

The DNAiTECH Gen-2 is a lightweight, portable instrument that integrates with Android smartphone, designed for performing isothermal amplification in any setting. 

Compared to the first generation, the DNAiTECH Gen-2 has been optimized and improved to offer a wider temperature range of 37 to 70 °C, making it possible to carry out all isothermal amplification methods within the device. With nine individually illuminated 0.2 mL PCR tube wells, users can test a range of conditions or samples between 10 and 100 μL in a single run. The device is also equipped with a filter for fluorescent dyes that have an excitation wavelength in the blue spectrum (470 nm) and emission in the green spectra, allowing real-time detection through the integrated smartphone app.

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The DNAiTECH is controlled via Bluetooth on an Android smartphone, with data displayed in real time on a dedicated app. This app also allows the user to export raw data as CSV files for replotting in alternative graphic forms. 

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Designed for both bench and field use, the DNAiTECH boasts a small footprint (weighing only 600 grams), low power requirements, and a rechargeable internal lithium-ion battery that allows multiple runs on a single charge. Additionally, the device can be powered or recharged with mains power using the supplied 15-volt charger or with the 12-volt vehicle adapter in the field.

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The DNAiTECH offers highly sensitive and rapid real-time quantitative nucleic acid analysis using isothermal methods in the temperature range of 37-70C. 

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The video recording shows the Generation 2 App in operation analyzing E. Coli samples with serial dilution E. Coli DNA calibration