S-TECH: subcellular particle isolation
S-TECH: DNAiTECH’s front-end DNA/RNA extraction technology
Isolation of urine subcellular particles
S-TECH is used to isolate subcellular particles using our track-etched polycarbonate membranes. The track-etched pores are highly uniform, and the membranes are low-binding because there is no traditional fibre membrane matrix to entrap particles. They are ideal for isolating subcellular particles such as exosomes and microvesicles. They also concentrate; they enable the removal of excess fluid and the recovery of particles for the next stage of their analysis. Buffer exchange is also easy using the S-TECH system, enabling the transfer of the recovered particles into the most appropriate reagent mix for secondary analysis.
When using S-TECH polycarbonate track etch membranes, it is important to prevent the sample from drying on the membrane.
See the video demonstration for isolation, concentration, and buffer exchange of urine particles followed by TRPS particle analysis.
Isolation of serum subcellular particles
Attached is a video demonstrating S-TECH's versatility in isolating subcellular particles from human serum. Our EV isolation membranes use geometrically precise track-etched pores in thin-film polycarbonate, a configuration that eliminates the entrapment of subcellular particles that occurs with fibre- or mesh-type filtration systems. In the video, we demonstrate how easy S-TECH is to use as a single device for the isolation, purification, concentration and buffer exchange of subcellular particles from 125 µL of serum (250ul total volume diluted in PBS 1:1). We also demonstrate the particle size distribution by tunable resistive pulse sensing (TRPS) and the efficient protein purification that occurs during the S-TECH processing of the serum sample.
