Technology Evolution

Gen 2 tube based isothermal system

The Generation 2 instrument is an evolution of our gen 1 hardware. Both Gen 1 and Gen 2 systems are tube based isothermal amplification devices. The Gen 2 device has a temperature range from 37 to 70C, supporting a variety of isothermal and CRISPR reactions. It has can 9 reactions positions for 0.2ml PCR tubes, Gen 2 is Li-ion battery powered for in-field operation, it is Bluetooth enabled, the operational app is installed on android phones, uses the phone camera to perform real-time fluorescence measurements. Gen 2 provides quantification of amplification using 3 or more calibration tubes for the target nucleic acid.

Gen 3.1 chip technology system

the Gen 3.1 hardware is our first instrument for our new chip technology. The hardware was developed in 2025 for the Senegal trial of our yellow fever and measles tests. The Gen 3.1 accepts chips with 4 independent reaction zones in a square reaction well array formation. the Gen 3.1 runs LAMP reactions first and then a secondary CRISPR reaction confirmation reaction is introduced. Gen 3.1 is battery powered, can run up to 4 days without recharging, it is lightweight and compact and designed for POC applications. Gen 3.1 is highly innovative and subject to multiple patent claims.

Gen 3.2 chip technology system

The Gen 3.2 hardware is our latest version of our POC systems, it integrates LAMP and CRISPR into a single step real-time capable diagnostic system. The first version of Gen3.2 is due in September 2026. The Gen 3.2 system has 8 reaction wells in a linear format and designed for multichannel pipette loading. Gen 3.2 is predecessor to Gen 3.3 which is due for release in 2027 and will have the optical systems for real-time processing. Gen 3.2/3 are to be battery powered with minimum of 1 day battery operation. Gen 3.2/3 will screen for multiple pathogens simultaneously, for example porcine pathogens (see Left). Hardware image not available at present.

Denaturator technology

The denaturator technology was developed for rapid processing of samples prior to the diagnostic test. The device is a battery powered rapid boiling device for heating samples rapidly to 90-100C with approximately 90 seconds. The samples are extracted in our lysis solution containing detergent, which ruptures cells and strips the proteins from RNA or DNA, making the nucleic acids accessible for diagnostic testing. The denaturator can process up to 75 individual samples on a single battery charge.

S-TECH Extraction technology

The S-TECH is DNAiTECH's extraction system developed for the extraction of nucleic acids after sample heating and cell rupture using the denaturator. The extraction of nucleic acids using S-TECH is described here.

S-TECH can also enable isolation of subcellular particles such as extracellular vesicles and micro-vesicles using our nano pore track etched polycarbonate membranes. Demonstration of S-TECH PCTE membranes to recover urinary and serum subcellular particles is shown here.