Auckland, March 16th 2021
S-Technology: subcellular particle isolation
Cloudy Bay, Marlborough
S-TECH is used to isolate subcellular particles using our track etched polycarbonate membranes. The track etched pores are highly uniform and the membranes are low binding, because there is no traditional fibre membrane matrix to entrap particles. They are ideal for isolation of subcellular particles such as exosomes and micro-vesicles. They are also concentrating, they enable the removal of excessive fluid and the recovery of particles for the next stage of their analysis. Buffer exchange is also easy using the S-TECH system, enabling the transfer of the recovered particles into the most appropriate reagent mix for secondary analysis.
When using S-TECH polycarbonate track etch membranes it is important to prevent the sample from going to dryness on the membrane.
See the video demonstration for isolation, concentration and buffer exchange of urine particles followed by TRPS particle analysis
Isolation of serum subcellular particles
Attached is a video demonstrating the versatility of S-TECH for the isolation of subcellular particles from human serum. Our EV isolation membranes use geometrically precise track-etched pores in thin film polycarbonate, a configuration which eliminates the entrapment of subcellular particles which occurs with fibre or mesh type filtration systems. In the video we demonstrate how easy S-TECH is to use as a single device for the isolation, purification, concentration and buffer exchange of subcellular particles from 125ul of serum (250ul total volume diluted in PBS 1:1). We also demonstrate the particle size distribution by tunable resistive pulse sensing (TRPS) and the efficient protein purification that occurs during the S-TECH processing of the serum sample.
Individual S-TECH unit on the right is a dispossible, single use component.
The S-TECH comes in boxes of 32 S-TECH units, four bases and four reservoirs plus the vacuum locking devices. The bases and the reservoirs are reusable, the reservoirs can be bleached, washed with milliQ water and used many times without cross contamination between samples
