INNOVATIONS FOR POC DIAGNOSTICS

DNAiTECH delivers molecular diagnostic testing for agritech, human health, and environmental applications. We have patented the integration of LAMP and CRISPR technologies for global point-of-care diagnostics. Our platform provides a complete solution, delivering results within 45 minutes from sample extraction and meeting all World Health Organisation ASSURED criteria. The sealed, integrated LAMP-CRISPR biochemistry eliminates contamination risk associated with two-step systems. Freeze-dried reagents in chips remove the need for cold-chain transport and storage, even in tropical climates. This integration ensures high sensitivity and specificity, while the CRISPR visual readout eliminates the need for expensive optical systems. Our S-TECH front-end extraction system enables archiving and transport of labile samples, such as RNA, without cold-chain requirements. For more information about our platform and the rationale for our approach, please see our white paper here 

Below is an example of our diagnostic chip technology in action. American foulbrood (AFB) is a serious bacterial infection of hives that requires the mandatory destruction of hives (in New Zealand). We have developed a rapid chip assay that detects Paenibacillus larvae in hive-entrance and brood-frame swabs. The linear chip assay below shows the analysis of four samples. In each case, the left lane detects the honeybee gene rps5, and the right lane tests for American foulbrood mtpR. Intense fluorescence indicates a positive reaction. All samples were positive for rps5, as expected, and only samples 2 and 3 showed AFB (mtpR). This test uses DNAiTECH’s Gen 3.2 linear chip technology with eight reaction wells.