
Using DNAiTECH
Real-time RNA/DNA measurements by DNAiTECH and Smartphone App

Real-time nucleic acid detection using DNAiTECH and the mobile phone is easy. The procedure is automated after the initial set-up phase, with little user input required from raw data collection to curve fitting and data analysis.
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The DNAiTECH instrument is operated via an app on the smartphone using a Bluetooth connection.
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The user can recall a previous program or establish a new program, defining the assay conditions, including reaction temperature, run time, initial delay settings, images per minute, the calibration concentration, and dilutions.
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The temperature range available is from 37-70°C. This temperature range covers all isothermal amplification assay methods.
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The DNAiTECH instrument allows for up to nine clear topped 0.2 ml PCR tubes.
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DNAiTECH kits use loop-mediated isothermal amplification.
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The PCR tubes for DNAiTECH LAMP assays contain all the test components, including buffer, magnesium salts, 4 or 6 specific LAMP primers, Bst strand displacing polymerase, nucleotides, Evergreen dye, sample and/or calibration DNA. The tubes are overlaid with mineral oil or a wax bead, which melts at the reaction temperature.
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Check out the video below of a recorded DNAiTECH assay and the specific steps involved in the assay process.

Video: Watch the video to see how DNAiTECH operates, including a 48-minute LAMP analysis of E. coli calibration standards and an unknown sample.
The data was collected using a Samsung Galaxy A10 smartphone.
In the video, you'll see the following steps:
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The user selects a program.
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DNAiTECH instrument equilibrates to the LAMP assay reaction temperature.
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Camera settings are optimized, including exposure and focus.
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Raw data is collected.
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Positive reactions are selected to be normalized to 100%.
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The curve fitting process is performed.
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The line is positioned for time to positivity (TP).
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Graphics show the processed LAMP assay curves, r-squared value, and calculated unknown sample concentration.
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Raw data can be exported as CSV files, and reprocessed by returning to the previous window.