Using DNAiTECH 

Real-time nucleic acid detection using DNAiTECH and the mobile phone is easy. The procedure is automated after the initial set-up phase, with little user input required from raw data collection to curve fitting and data analysis.  

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• The DNAiTECH instrument is operated via an app on the smartphone using a Bluetooth connection. ​

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• The user can recall a previous program or establish a new program, defining the assay conditions, including reaction temperature, run time, initial delay settings, images per minute, the calibration concentration, and dilutions.

 

• The temperature range available is from 37-70°C. This temperature range covers all isothermal amplification assay methods. 

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• The DNAiTECH instrument allows for up to nine clear topped 0.2 ml PCR tubes.

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• DNAiTECH kits use loop-mediated isothermal amplification.

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• The PCR tubes for DNAiTECH LAMP assays contain all the test components, including buffer, magnesium salts, 4 or 6 specific LAMP primers, Bst strand displacing polymerase, nucleotides, Evergreen dye,  sample and/or calibration DNA. The tubes are overlaid with mineral oil or a wax bead, which melts at the reaction temperature.

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• Check out the video below of a recorded DNAiTECH assay and the specific steps involved in the assay process.