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Real-time RNA/DNA measurements by DNAiTECH and Smartphone App

Portable isothermal amplification using DNAiTECH instrument with a smartphone.

Real-time nucleic acid detection using DNAiTECH and the mobile phone is easy. The procedure is automated after the initial set-up phase, with little user input required from raw data collection to curve fitting and data analysis.  

  • The DNAiTECH instrument is operated via an app on the smartphone using a Bluetooth connection. ​

  • The user can recall a previous program or establish a new program, defining the assay conditions, including reaction temperature, run time, initial delay settings, images per minute, the calibration concentration, and dilutions.


  • The temperature range available is from 37-70°C. This temperature range covers all isothermal amplification assay methods. 

  • The DNAiTECH instrument allows for up to nine clear topped 0.2 ml PCR tubes.

  • DNAiTECH kits use loop-mediated isothermal amplification.

  • The PCR tubes for DNAiTECH LAMP assays contain all the test components, including buffer, magnesium salts, 4 or 6 specific LAMP primers, Bst strand displacing polymerase, nucleotides, Evergreen dye,  sample and/or calibration DNA. The tubes are overlaid with mineral oil or a wax bead, which melts at the reaction temperature.

  • Check out the video below of a recorded DNAiTECH assay and the specific steps involved in the assay process.

Video: Watch the video to see how DNAiTECH operates, including a 48-minute LAMP analysis of E. coli calibration standards and an unknown sample.

The data was collected using a Samsung Galaxy A10 smartphone.

In the video, you'll see the following steps:

  1. The user selects a program.

  2. DNAiTECH instrument equilibrates to the LAMP assay reaction temperature.

  3. Camera settings are optimized, including exposure and focus.

  4. Raw data is collected.

  5. Positive reactions are selected to be normalized to 100%.

  6. The curve fitting process is performed.

  7. The line is positioned for time to positivity (TP).

  8. Graphics show the processed LAMP assay curves, r-squared value, and calculated unknown sample concentration.

  9. Raw data can be exported as CSV files, and reprocessed by returning to the previous window.

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