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Using DNAiTECH

Using DNAiTECH 

Real-time nucleic acid measurements by DNAiTECH Gen2 and the Smartphone App

Portable isothermal amplification using DNAiTECH instrument with a smartphone.

In 2024 we will release Gen3, our microfluidic system for POC applications. The Gen3 system will achieve the WHO ASSURED criteria for POC diagnostics.

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The current commercial DNAiTECH instrument is the Gen2 system. The Gen 2 instrument can be used in the field with its internal Li-ion battery, it is a tube based system.

 

Using the Gen2 DNAiTECH system and the mobile phone is easy. The procedure is automated after the initial set-up phase, with little user input required from raw data collection to curve fitting and data analysis.  

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  • The DNAiTECH instrument is operated via an app on the smartphone using a Bluetooth connection. ​

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  • The user can recall a previous program or establish a new program, defining the assay conditions, including reaction temperature, run time, initial delay settings, images per minute, the calibration concentration, and dilutions.

 

  • The temperature range available is from 37-70°C. This temperature range covers all isothermal amplification assay methods. 

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  • The DNAiTECH Gen 2 instrument allows for up to nine clear topped 0.2 ml PCR tubes.​​

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  • Check out the video below of a recorded DNAiTECH assay and the specific steps involved in the assay process.

Video: Watch the video to see how DNAiTECH operates, including a 48-minute LAMP analysis of E. coli calibration standards and an unknown sample.

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The data was collected using a Samsung Galaxy A10 smartphone.

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In the video, you'll see the following steps:

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  1. The user selects a program.

  2. DNAiTECH instrument equilibrates to the LAMP assay reaction temperature.

  3. Camera settings are optimized, including exposure and focus.

  4. Raw data is collected.

  5. Positive reactions are selected to be normalized to 100%.

  6. The curve fitting process is performed.

  7. The line is positioned for time to positivity (TP).

  8. Graphics show the processed LAMP assay curves, r-squared value, and calculated unknown sample concentration.

  9. Raw data can be exported as CSV files, and reprocessed by returning to the previous window.

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